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competent e coli neb10 beta cells  (New England Biolabs)


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    New England Biolabs competent e coli neb10 beta cells
    Competent E Coli Neb10 Beta Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/competent+e+coli+neb10+beta+cells/NEB+10-beta+Competent+E%2E+coli/pm41638488-73-7-12
    Average 99 stars, based on 1376 article reviews
    competent e coli neb10 beta cells - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Characterization of an aromatic O-prenyltransferase from the edible mushroom Sparassis crispa.
    Article Snippet: Next, the ScPTMY encoding DNA sequence was synthesized by Twist Bioscience (South San Francisco, CA, USA) after being codon-optimized for expression in E. coli using their bioinformatic tool (Table S2).

    Purification:

    Article Title: Secreted lipases from Malassezia globosa: recombinant expression and determination of their substrate specificities.
    Article Snippet: PCR was performed using KOD Hot Start Master Mix (Merck) and 2 μl genomic DNA as the template according to the manufacturer’s specification. .. Purified PCR products and pPIC3.5 were digested with BamHI or EcoRI and NotI (recognition sites are underlined in the primer sequences), ligated with T4 DNA Ligase (New England Biolabs) and used to transform competent E. coli NEB10-beta cells. ..

    Polymerase Chain Reaction:

    Article Title: Secreted lipases from Malassezia globosa: recombinant expression and determination of their substrate specificities.
    Article Snippet: PCR was performed using KOD Hot Start Master Mix (Merck) and 2 μl genomic DNA as the template according to the manufacturer’s specification. .. Purified PCR products and pPIC3.5 were digested with BamHI or EcoRI and NotI (recognition sites are underlined in the primer sequences), ligated with T4 DNA Ligase (New England Biolabs) and used to transform competent E. coli NEB10-beta cells. ..



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    ( A ) Cholesterol levels in islets extracted from mice fed a chow vs high cholesterol (high chol) diet for 5 wk; n=3. ( B ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose ( G6 ) media followed by 100 μM isobutyl methylxanthine (IBMX) +10 µM Forskolin (FSK) for maximal response; area under the curve (AUC) for Ex-4 stimulation period shown; n=4. ( C ) Percentage of insulin secretion from mouse islets preincubated with Veh or methyl-β-cyclodextrin (MβCD) loaded with 20 mM cholesterol (MβCD/chol) for 1 hr before stimulation with a secretagogue cocktail (20 mM glucose, 30 mM KCl, 10 µM FSK and 100 µM IBMX) for 30 min; n=3. ( D ) Representative images from raster image correlation spectroscopy (RICS) analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or MβCD/chol for 1 hr before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( E ) Average RICS diffusion coefficient in cells from ( D ) (n=4). ( F ) cAMP responses in islets isolated from Pdx1 Cre-ERT CAMPER mice (expressing the cAMP reporter T EPAC VV in <t>beta</t> cells) preincubated with control (Veh) or lipoprotein-deficient serum (LPDS) media supplemented with 10 µM simvastatin (LPDS/simv) overnight and stimulated with 100 nM Ex-4 in G6 followed by 16 mM glucose (G16) and 100 μM IBMX +10 µM FSK for maximal response; AUC for Ex-4 stimulation period shown; n=6. ( G ) Representative images from RICS analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or LPDS/simv media overnight before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( H ) Average RICS diffusion coefficient in cells from ( G ) (n=5). Data is mean +/- SEM; *p<0.05 by paired t-test or one-way ANOVA with Sidak’s multiple comparison test.
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    ( A ) Cholesterol levels in islets extracted from mice fed a chow vs high cholesterol (high chol) diet for 5 wk; n=3. ( B ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose ( G6 ) media followed by 100 μM isobutyl methylxanthine (IBMX) +10 µM Forskolin (FSK) for maximal response; area under the curve (AUC) for Ex-4 stimulation period shown; n=4. ( C ) Percentage of insulin secretion from mouse islets preincubated with Veh or methyl-β-cyclodextrin (MβCD) loaded with 20 mM cholesterol (MβCD/chol) for 1 hr before stimulation with a secretagogue cocktail (20 mM glucose, 30 mM KCl, 10 µM FSK and 100 µM IBMX) for 30 min; n=3. ( D ) Representative images from raster image correlation spectroscopy (RICS) analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or MβCD/chol for 1 hr before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( E ) Average RICS diffusion coefficient in cells from ( D ) (n=4). ( F ) cAMP responses in islets isolated from Pdx1 Cre-ERT CAMPER mice (expressing the cAMP reporter T EPAC VV in <t>beta</t> cells) preincubated with control (Veh) or lipoprotein-deficient serum (LPDS) media supplemented with 10 µM simvastatin (LPDS/simv) overnight and stimulated with 100 nM Ex-4 in G6 followed by 16 mM glucose (G16) and 100 μM IBMX +10 µM FSK for maximal response; AUC for Ex-4 stimulation period shown; n=6. ( G ) Representative images from RICS analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or LPDS/simv media overnight before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( H ) Average RICS diffusion coefficient in cells from ( G ) (n=5). Data is mean +/- SEM; *p<0.05 by paired t-test or one-way ANOVA with Sidak’s multiple comparison test.
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    ( A ) Cholesterol levels in islets extracted from mice fed a chow vs high cholesterol (high chol) diet for 5 wk; n=3. ( B ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose ( G6 ) media followed by 100 μM isobutyl methylxanthine (IBMX) +10 µM Forskolin (FSK) for maximal response; area under the curve (AUC) for Ex-4 stimulation period shown; n=4. ( C ) Percentage of insulin secretion from mouse islets preincubated with Veh or methyl-β-cyclodextrin (MβCD) loaded with 20 mM cholesterol (MβCD/chol) for 1 hr before stimulation with a secretagogue cocktail (20 mM glucose, 30 mM KCl, 10 µM FSK and 100 µM IBMX) for 30 min; n=3. ( D ) Representative images from raster image correlation spectroscopy (RICS) analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or MβCD/chol for 1 hr before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( E ) Average RICS diffusion coefficient in cells from ( D ) (n=4). ( F ) cAMP responses in islets isolated from Pdx1 Cre-ERT CAMPER mice (expressing the cAMP reporter T EPAC VV in <t>beta</t> cells) preincubated with control (Veh) or lipoprotein-deficient serum (LPDS) media supplemented with 10 µM simvastatin (LPDS/simv) overnight and stimulated with 100 nM Ex-4 in G6 followed by 16 mM glucose (G16) and 100 μM IBMX +10 µM FSK for maximal response; AUC for Ex-4 stimulation period shown; n=6. ( G ) Representative images from RICS analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or LPDS/simv media overnight before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( H ) Average RICS diffusion coefficient in cells from ( G ) (n=5). Data is mean +/- SEM; *p<0.05 by paired t-test or one-way ANOVA with Sidak’s multiple comparison test.
    Purification High Efficiency Neb10 Beta Competent E Coli Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Cholesterol levels in islets extracted from mice fed a chow vs high cholesterol (high chol) diet for 5 wk; n=3. ( B ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose ( G6 ) media followed by 100 μM isobutyl methylxanthine (IBMX) +10 µM Forskolin (FSK) for maximal response; area under the curve (AUC) for Ex-4 stimulation period shown; n=4. ( C ) Percentage of insulin secretion from mouse islets preincubated with Veh or methyl-β-cyclodextrin (MβCD) loaded with 20 mM cholesterol (MβCD/chol) for 1 hr before stimulation with a secretagogue cocktail (20 mM glucose, 30 mM KCl, 10 µM FSK and 100 µM IBMX) for 30 min; n=3. ( D ) Representative images from raster image correlation spectroscopy (RICS) analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or MβCD/chol for 1 hr before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( E ) Average RICS diffusion coefficient in cells from ( D ) (n=4). ( F ) cAMP responses in islets isolated from Pdx1 Cre-ERT CAMPER mice (expressing the cAMP reporter T EPAC VV in beta cells) preincubated with control (Veh) or lipoprotein-deficient serum (LPDS) media supplemented with 10 µM simvastatin (LPDS/simv) overnight and stimulated with 100 nM Ex-4 in G6 followed by 16 mM glucose (G16) and 100 μM IBMX +10 µM FSK for maximal response; AUC for Ex-4 stimulation period shown; n=6. ( G ) Representative images from RICS analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or LPDS/simv media overnight before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( H ) Average RICS diffusion coefficient in cells from ( G ) (n=5). Data is mean +/- SEM; *p<0.05 by paired t-test or one-way ANOVA with Sidak’s multiple comparison test.

    Journal: eLife

    Article Title: Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cells

    doi: 10.7554/eLife.101011

    Figure Lengend Snippet: ( A ) Cholesterol levels in islets extracted from mice fed a chow vs high cholesterol (high chol) diet for 5 wk; n=3. ( B ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose ( G6 ) media followed by 100 μM isobutyl methylxanthine (IBMX) +10 µM Forskolin (FSK) for maximal response; area under the curve (AUC) for Ex-4 stimulation period shown; n=4. ( C ) Percentage of insulin secretion from mouse islets preincubated with Veh or methyl-β-cyclodextrin (MβCD) loaded with 20 mM cholesterol (MβCD/chol) for 1 hr before stimulation with a secretagogue cocktail (20 mM glucose, 30 mM KCl, 10 µM FSK and 100 µM IBMX) for 30 min; n=3. ( D ) Representative images from raster image correlation spectroscopy (RICS) analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or MβCD/chol for 1 hr before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( E ) Average RICS diffusion coefficient in cells from ( D ) (n=4). ( F ) cAMP responses in islets isolated from Pdx1 Cre-ERT CAMPER mice (expressing the cAMP reporter T EPAC VV in beta cells) preincubated with control (Veh) or lipoprotein-deficient serum (LPDS) media supplemented with 10 µM simvastatin (LPDS/simv) overnight and stimulated with 100 nM Ex-4 in G6 followed by 16 mM glucose (G16) and 100 μM IBMX +10 µM FSK for maximal response; AUC for Ex-4 stimulation period shown; n=6. ( G ) Representative images from RICS analysis of GLP-1R plasma membrane lateral diffusion in INS-1 832/3 SNAP/FLAG-hGLP-1R cells preincubated with Veh or LPDS/simv media overnight before labelling with SNAP-Surface Alexa Fluor 647 and stimulation with Veh or 100 nM Ex-4. ( H ) Average RICS diffusion coefficient in cells from ( G ) (n=5). Data is mean +/- SEM; *p<0.05 by paired t-test or one-way ANOVA with Sidak’s multiple comparison test.

    Article Snippet: Digested DNA samples were transformed into NEB10-beta Competent E. coli High Efficiency cells (C3019H, New England Biolabs) according to the manufacturer’s instructions.

    Techniques: Isolation, Transduction, Spectroscopy, Membrane, Diffusion-based Assay, Expressing, Control, Comparison